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rtstartm trf & tirna pretreatment kit as-fs-005  (Arraystar inc)

 
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    Structured Review

    Arraystar inc rtstartm trf & tirna pretreatment kit as-fs-005
    Rtstartm Trf & Tirna Pretreatment Kit As Fs 005, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rtstartm+trf+and+tirna+pretreatment+kit+as-fs-005/rtstartm+trf+tirna+pretreatment+kit/pmc11914182-278-22-28
    Average 90 stars, based on 1 article reviews
    rtstartm trf & tirna pretreatment kit as-fs-005 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Ligation:

    Article Title: Expression Profiles of tRNA-Derived Small RNAs and Their Potential Roles in Primary Nasopharyngeal Carcinoma.
    Article Snippet: Before the sequencing experiment, we checked each RNA sample’s integrity and quantity using agarose gel electrophoresis and a NanodropTM instrument (ND-1000, Thermo Fisher Scientific, DE, United States). tsRNAs are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. .. We do the following treatments using a rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar Inc., MD, United States) before library preparation for total RNA samples: 3′-aminoacyl (charged) deacylation to 3′-OH for 3′adaptor ligation, 3′-cP (2′,3′-cyclic Frontiers in Molecular Biosciences | www.frontiersin.org December 2021 | Volume 8 | Article 7806212 phosphate) removal to 3′-OH for 3′adaptor ligation, 5′-OH (hydroxyl group) phosphorylation to 5′-P for 5′-adaptor ligation, and m1A and m3C demethylation for efficient reverse transcription. cDNA was then synthesized and amplified using Illumina’s proprietary RT primers and amplification primers. ..

    Phospho-proteomics:

    Article Title: Expression Profiles of tRNA-Derived Small RNAs and Their Potential Roles in Primary Nasopharyngeal Carcinoma.
    Article Snippet: Before the sequencing experiment, we checked each RNA sample’s integrity and quantity using agarose gel electrophoresis and a NanodropTM instrument (ND-1000, Thermo Fisher Scientific, DE, United States). tsRNAs are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. .. We do the following treatments using a rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar Inc., MD, United States) before library preparation for total RNA samples: 3′-aminoacyl (charged) deacylation to 3′-OH for 3′adaptor ligation, 3′-cP (2′,3′-cyclic Frontiers in Molecular Biosciences | www.frontiersin.org December 2021 | Volume 8 | Article 7806212 phosphate) removal to 3′-OH for 3′adaptor ligation, 5′-OH (hydroxyl group) phosphorylation to 5′-P for 5′-adaptor ligation, and m1A and m3C demethylation for efficient reverse transcription. cDNA was then synthesized and amplified using Illumina’s proprietary RT primers and amplification primers. ..

    Reverse Transcription:

    Article Title: Expression Profiles of tRNA-Derived Small RNAs and Their Potential Roles in Primary Nasopharyngeal Carcinoma.
    Article Snippet: Before the sequencing experiment, we checked each RNA sample’s integrity and quantity using agarose gel electrophoresis and a NanodropTM instrument (ND-1000, Thermo Fisher Scientific, DE, United States). tsRNAs are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. .. We do the following treatments using a rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar Inc., MD, United States) before library preparation for total RNA samples: 3′-aminoacyl (charged) deacylation to 3′-OH for 3′adaptor ligation, 3′-cP (2′,3′-cyclic Frontiers in Molecular Biosciences | www.frontiersin.org December 2021 | Volume 8 | Article 7806212 phosphate) removal to 3′-OH for 3′adaptor ligation, 5′-OH (hydroxyl group) phosphorylation to 5′-P for 5′-adaptor ligation, and m1A and m3C demethylation for efficient reverse transcription. cDNA was then synthesized and amplified using Illumina’s proprietary RT primers and amplification primers. ..

    Synthesized:

    Article Title: Expression Profiles of tRNA-Derived Small RNAs and Their Potential Roles in Primary Nasopharyngeal Carcinoma.
    Article Snippet: Before the sequencing experiment, we checked each RNA sample’s integrity and quantity using agarose gel electrophoresis and a NanodropTM instrument (ND-1000, Thermo Fisher Scientific, DE, United States). tsRNAs are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. .. We do the following treatments using a rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar Inc., MD, United States) before library preparation for total RNA samples: 3′-aminoacyl (charged) deacylation to 3′-OH for 3′adaptor ligation, 3′-cP (2′,3′-cyclic Frontiers in Molecular Biosciences | www.frontiersin.org December 2021 | Volume 8 | Article 7806212 phosphate) removal to 3′-OH for 3′adaptor ligation, 5′-OH (hydroxyl group) phosphorylation to 5′-P for 5′-adaptor ligation, and m1A and m3C demethylation for efficient reverse transcription. cDNA was then synthesized and amplified using Illumina’s proprietary RT primers and amplification primers. ..

    Amplification:

    Article Title: Expression Profiles of tRNA-Derived Small RNAs and Their Potential Roles in Primary Nasopharyngeal Carcinoma.
    Article Snippet: Before the sequencing experiment, we checked each RNA sample’s integrity and quantity using agarose gel electrophoresis and a NanodropTM instrument (ND-1000, Thermo Fisher Scientific, DE, United States). tsRNAs are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. .. We do the following treatments using a rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar Inc., MD, United States) before library preparation for total RNA samples: 3′-aminoacyl (charged) deacylation to 3′-OH for 3′adaptor ligation, 3′-cP (2′,3′-cyclic Frontiers in Molecular Biosciences | www.frontiersin.org December 2021 | Volume 8 | Article 7806212 phosphate) removal to 3′-OH for 3′adaptor ligation, 5′-OH (hydroxyl group) phosphorylation to 5′-P for 5′-adaptor ligation, and m1A and m3C demethylation for efficient reverse transcription. cDNA was then synthesized and amplified using Illumina’s proprietary RT primers and amplification primers. ..

    Construct:

    Article Title: tsRNA-00764 Regulates Estrogen and Progesterone Synthesis and Lipid Deposition by Targeting PPAR-γ in Duck Granulosa Cells
    Article Snippet: RNA was extracted from granulosa cells using TRIzol (Thermo Scientific, Waltham, MA, USA), and RNA quality was assessed using Tape Station 2200 (Agilent Technologies, Santa Clara, CA, USA). .. We first removed RNA modifications and constructed the small RNA-seq libraries with the rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar, Rockville, MD, USA). .. Then, tsRNA sequencing and expression quantification were, respectively, performed using NextSeq 500 (Illumina, San Diego, CA, USA) and Bioanalyzer (Agilent Technologies, California, CA, USA) at Kangcheng Biological Company (Shanghai, China).

    RNA Sequencing:

    Article Title: tsRNA-00764 Regulates Estrogen and Progesterone Synthesis and Lipid Deposition by Targeting PPAR-γ in Duck Granulosa Cells
    Article Snippet: RNA was extracted from granulosa cells using TRIzol (Thermo Scientific, Waltham, MA, USA), and RNA quality was assessed using Tape Station 2200 (Agilent Technologies, Santa Clara, CA, USA). .. We first removed RNA modifications and constructed the small RNA-seq libraries with the rtStarTM tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar, Rockville, MD, USA). .. Then, tsRNA sequencing and expression quantification were, respectively, performed using NextSeq 500 (Illumina, San Diego, CA, USA) and Bioanalyzer (Agilent Technologies, California, CA, USA) at Kangcheng Biological Company (Shanghai, China).

    RNA modification:

    Article Title: tRNA-derived fragment tRF-1001: A novel anti-angiogenic factor in pathological ocular angiogenesis
    Article Snippet: RNA integrity was evaluated using NanoDrop ND-1000 Spectrophotometer (Agilent, USA) and the concentration of RNAs was determined using the spectrophotometer at 260 nm. .. To quantify tRF-1001 level, the rtStarTM tRF and tiRNA pretreatment kit (AS-FS-005, Arraystar, USA) was employed to remove RNA modification. .. The rtStarTM First-strand cDNA Synthesis kit (AS-FS-003, Arraystar, USA) was then used to reversely transcribe RNAs into cDNAs.



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